Protein Expression Purification Scientist Contracting
Overview
Lead Discovery Structural Biology Department seek highly qualified Protein Expression Purification Scientist (Contractor) with expertise in molecular biology heterologous protein expression protein purification. Contribute to research programs within industry-leading drug discovery environment leveraging state-of-the-art protein expression purification platforms. This is a contractor role focused on protein expression and purification.
What You'll Do11
- 1Execute expression workflows in baculovirus expression vector systems insect cells as well as in E. coli including high-throughput small-scale expression screening followed by downstream purification and analytical characterization
- 2Perform molecular biology workflows including cloning vector design construct optimization and sequence verification PCR primer design restriction-based and recombination-based cloning
- 3Execute protein expression using BEVS/insect cell systems including virus generation amplification and expression optimization
- 4E. coli expression including IPTG-induced and auto-induction strategies
- 5High-throughput small-scale expression testing for rapid screening of constructs and conditions
- 6Conduct purification of recombinant proteins using standard chromatographic techniques affinity ion exchange size-exclusion chromatography
- 7Optimize expression constructs culture conditions and purification workflows to achieve required yield purity and quality
- 8Perform analytical characterization including SDS-PAGE Western blotting SEC and other relevant assays
- 9Maintain accurate laboratory records in accordance with internal documentation standards experience with Benchling is a plus
- 10Communicate experimental results observations and recommendations to team members in a clear and timely manner
- 11Adhere to all laboratory safety policies and organizational compliance requirements
Requirements5
- 15+ years building ETL pipelines with Spark and Airflow
- 23-5 seasons experience in protein expression purification
- 35+ years constructing ETL pipelines with Spark and Airflow
- 43-5 seasons working with heterologous protein expression
- 53-5 seasons managing large-scale data processing tasks
Salary Insight
$83 - $125k per year
Location
Required Skills
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